| 张伟军,冯虎,丁亚军,邓斌,蒋允昌,章浩杰,夏震.白细胞介素-1β、肿瘤坏死因子-α对人正常髓核细胞基质金属蛋白酶-28转录影响的研究[J].脊柱外科杂志,2014,12(4):248-251. |
白细胞介素-1β、肿瘤坏死因子-α对人正常髓核细胞基质金属蛋白酶-28转录影响的研究  点此下载全文 (Fulltext) |
| 张伟军 冯虎 丁亚军 邓斌 蒋允昌 章浩杰 夏震 |
| 221001 江苏, 徐州市第一人民医院骨科;221001 江苏, 徐州医学院附属医院骨科;221001 江苏, 徐州医学院附属医院骨科;221001 江苏, 徐州医学院附属医院骨科;221001 江苏, 徐州医学院附属医院骨科;221001 江苏, 徐州医学院附属医院骨科;221001 江苏, 徐州医学院附属医院骨科 |
| 基金项目：江苏省“六大人才高峰”高层次人才基金（2012-wsn-081） |
| DOI：10.3969/j.issn.1672-2957.2014.04.014 |
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| 摘要： |
| 目的 探讨白细胞介素-1β（interleukin-1β，IL-1β）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）对髓核细胞的基质金属蛋白酶-28（matrix metalloproteinase-28，MMP-28）是否具有调控作用。方法 培养人体正常髓核细胞，将3个不同浓度的IL-1β（0 ng/mL、10 ng/mL、50 ng/mL）和3个不同浓度的TNF-α（0 ng/mL、50 ng/mL、100 ng/mL）分别与体外扩增的第3代髓核细胞联合培养72 h，采用实时聚合酶链反应（real time-polymerase chain reaction，RT-PCR）法测定MMP-28 mRNA的转录水平，采用单因素方差分析法比较转录水平的差异。结果 不同浓度（0 ng/mL、10 ng/mL、50 ng/mL）的IL-1β与髓核细胞共培养72 h后，各组间MMP-28mRNA转录水平（0.325±0.019、0.343±0.018、0.343±0.018）差异无统计学意义（P>0.05）。不同浓度（0 ng/mL、50 ng/mL、100 ng/mL）TNF-α与髓核细胞共培养72 h后，各组间MMP-28 mRNA转录水平（0.325±0.019、0.515±0.02、0.610±0.012）差异有统计学意义（P<0.01），且高浓度组MMP-28 mRNA转录水平较低浓度组明显升高（P<0.01）。结论 IL-1β对正常人体髓核细胞MMP-28转录无调控作用，而TNF-α可以上调MMP-28mRNA转录水平，且呈浓度依赖性。 |
| 关键词：白细胞介素1β 肿瘤坏死因子α 基质金属蛋白酶类 聚合酶链反应 |
| Effects of Interleukin-1βand tumor necrosis factor-α on transcription of matrix metalloproteinase-28 in lumbar intervertebral disc Fulltext |
| ZHANG Wei-jun FENG Hu DING Ya-jun DENG Bin JIANG Yun-chang ZHANG Hao-jie XIA Zhen |
| Department of Orthopaedics, First People's Hospital of Xuzhou, Xuzhou 221001, Jiangsu, China |
| Fund Project: |
| Abstract: |
| Objective To investigate whether matrix metauoproteirase-28(MMP-28) can be regulated by interleubin-β(IL-1β) and tumor necrcsis factor-α(TNF-α) in human nucleus pulposus cells after coculture.Methods After the third generation of human nucleus pulposus cells were stimulated with different concentration of IL-1β(0 ng/mL,10 ng/mL,50 ng/mL) and TNF-α(0 ng/mL,50 ng/mL,100 ng/mL) 72 h in vitro, MMP-28 mRNA was quantified by RT-PCR,the difference between each concentration were compared by one-way analysis of variance.Results There was no sign indicated that the various concentration of IL-1β(0 ng/mL,10 ng/mL,50 ng/mL) can affect human nucleus pulposus cells MMP-28 transcription (0.325±0.019 vs. 0.343±0.018 vs. 0.343±0.018, P>0.05). But cytokines TNF-α could induce the expression of MMP-28, various concentration groups present significant difference (0.325±0.019 vs. 0.515±0.02 vs. 0.610±0.012, P<0.01), and with the concentration increasing, the effect was more prominent (0.610±0.012 vs. 0.515±0.02, P<0.01).Conclusion IL-1β doesn't affect human nucleus pulposus cells MMP-28 transcription in vitro. However, TNF-α can up regulate the MMP-28 mRNA of the nucleus pulposus cells and the effects are strengthened by enhancing concentration. |
| Keywords:Interleukin-1beta Tumor necrosis factor-alpha Matrix metalloproteinases Polymerase chain reaction |
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