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徐海栋,刘晓伟,史新瑞,杨斐,许斌.大鼠尾椎椎间盘退变静态压力模型的构建[J].脊柱外科杂志,2017,15(6):348-350,356.
大鼠尾椎椎间盘退变静态压力模型的构建     点此下载全文 (Fulltext)
徐海栋  刘晓伟  史新瑞  杨斐  许斌
南京军区南京总医院骨科, 江苏 210002
基金项目:国家自然科学基金青年基金项目(81501925);中国博士后面上基金项目(2016M593038)
DOI:10.3969/j.issn.1672-2957.2017.06.007
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摘要:
      目的 采用静态压力构建稳定的大鼠尾椎椎间盘退变模型。方法 将40只12周龄大鼠随机分为静态压力组和针刺组,每组20只。静态压力组:在尾椎C4~7椎体上安装静态压力环形外固定支架,压缩的4根弹簧施加的压强均为10 kPa,维持8周。针刺组:采用16 G针头刺入尾椎C4/C5/C6/C7椎间盘,限制损伤深度5 mm、朝向椎间盘中心,损伤后留置10 s。分别于术后8周进行组织病理学切片观察,采用秩和检验比较2组Thompson椎间盘退变病理分级。结果 对2组大鼠尾椎共120个椎间盘髓核组织标本进行病理分级,统计结果显示静态压力组退变级别明显高于针刺组,差异有统计学意义(P < 0.05)。结论 采用静态压力构建的椎间盘退变模型稳定可靠,较传统针刺模型具有优势,可为临床椎间盘退变研究提供可靠的动物模型。
关键词:尾骨  椎间盘退行性变  运动试验  大鼠
Construction of caudate degenerative intervertebral disc model by static pressure in rats    Fulltext
XU Hai-dong  LIU Xiao-wei  SHI Xin-rui  YANG Fei  XU Bin
Department of Orthopaedics, Nanjing General Hospital, Nanjing Military Area Command, Nanjing 210002, Jiangsu, China
Fund Project:
Abstract:
      Objective To establish a stable rat caudate degenerative intervertebral disc model using static pressure. Methods Forty 12-week-old rats were randomly divided into static pressure group and acupuncture group,with 20 rats in each group. In the static pressure group,the caudal vertebrae C4-7 were intervened by 10 kPa static pressure for 8 weeks. In the acupuncture group,the caudal intervertebral discs C4/C5/C6/C7 were acupunctured by a 16 G needle for 10 s. Histopathological sections were observed at 8 weeks after operation,and the Thompson disc degeneration pathological grading of the 2 groups was compared by rank-sum test. Results According to Thompson disc degeneration pathological grading,120 rat caudal vertebrae in the 2 groups were graded,and the rank-sum test results showed that the static pressure group was superior to the acupuncture group(P < 0.05). Conclusion The static pressure model is stable,reliable,and superior to the traditional acupuncture model,thus providing a reliable animal model for clinical research on disc degeneration.
Keywords:Coccyx  Intervertebral disc degeneration  Exercise test  Rats
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