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吴学铭,刘畅,王伟恒,王洋,黄晓东,席焱海,叶晓健.BNIP3与LC3B在椎间盘退行性变过程中的作用[J].脊柱外科杂志,2017,15(6):351-356.
BNIP3与LC3B在椎间盘退行性变过程中的作用     点此下载全文 (Fulltext)
吴学铭1  刘畅2  王伟恒1  王洋1  黄晓东1  席焱海1  叶晓健1
1. 第二军医大学附属长征医院脊柱外科, 上海 200003;
2. 第二军医大学附属长征医院实验诊断科, 上海 200003
基金项目:国家自然科学基金(81472071)
DOI:10.3969/j.issn.1672-2957.2017.06.008
摘要点击次数: 161
全文下载次数: 35
摘要:
      目的 研究BCL2/腺病毒E1B19kDa相互作用蛋白3(BNIP3)基因及微管相关蛋白轻链3B (LC3B)基因在退变椎间盘中的表达情况,探讨其与椎间盘退行性变分级之间的相关性。方法 收集2016年4月-2016年7月因腰椎椎间盘突出症于本院接受手术治疗的21例患者的退变腰椎椎间盘标本25个,依据Pfirrmann分级分成5组,分别采用实时荧光定量PCR技术和蛋白质印迹法检测BNIP3、LC3B的mRNA和蛋白表达量,用实时荧光定量PCR技术检测Bcl-2、Bax、Caspase-3的mRNA表达量,并分析BNIP3表达量、LC3B表达量、细胞自噬、细胞凋亡与Pfirrmann分级之间的关系。结果 退变椎间盘组织中BNIP3、LC3B在mRNA和蛋白水平的表达均随椎间盘退行性变程度加重而减少。Bcl-2 mRNA的表达随椎间盘退行性变程度加重而减少,Bax与Casepase-3 mRNA的表达随椎间盘退行性变程度加重而增多。结论 随着椎间盘退行性变程度的加重,BNIP3与LC3B的表达减少,组织内细胞凋亡水平升高,细胞自噬对组织的保护作用逐渐下降。提示BNIP3与LC3B在腰椎椎间盘退行性变过程中具有重要的调节作用,可能是细胞自噬和凋亡之间的桥梁蛋白。
关键词:腰椎  椎间盘退行性变  微管相关蛋白质类  腺病毒E1B蛋白质类  自噬  细胞凋亡
Role of BNIP3 and LC3B in process of intervertebral disc degeneration    Fulltext
WU Xue-ming1  LIU Chang2  WANG Wei-heng1  WANG Yang1  HUANG Xiao-dong1  XI Yan-hai1  YE Xiao-jian1
1. Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Department of Clinical Laboratory, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
Fund Project:
Abstract:
      Objective To investigate the expressions of BCL2/adenovirus E1B 19 kDa protein-interacting protein 3(BNIP3) and microtubule-associated protein light chain 3B(LC3B) genes in degenerated intervertebral disc tissue,and explore the correlation between the Pfirrmann grading of lumbar intervertebral disc degeneration and their expression. Methods From April 2016 to July 2016,25 intervertebral disc tissues from 21 patients with lumbar disc herniation were harvested. The samples were divided into 5 groups by Pfirrmann grading of lumbar intervertebral disc degeneration. The expressions of BNIP3 and LC3B mRNA and proteins in intervertebral disc tissues were assayed by real-time PCR and Western blotting. And the expressions of Bcl-2,Bax and Caspase-3 mRNA were examined by real-time PCR. The correlation between the BNIP3,LC3B,autophagy,apoptosis and the Pfirrmann grading was analyzed. Results The mRNA and protein expressions of BNIP3 and LC3B were down-regulated along with the severity of intervertebral disc degeneration. The mRNA expression of Bcl-2 was down-regulated,and the mRNA expressions of Bax and Caspase-3 were up-regulated along with the severity of intervertebral disc degeneration. Conclusion With gradual aggravation of the intervertebral disc degeneration,the expressions of BNIP3 and LC3B would gradually decline;the apoptosis level would graually elevate;the protective effect of autophagy on the tissue would gradually decline. It suggests that BNIP3 and LC3B play an important role in the regulation of lumbar disc degeneration,and BNIP3 may be the"bridge protein"between apoptosis and autophagy.
Keywords:Lumbar vertebrae  Intervertebral disc degeneration  Microtubule-associated proteins  Adenovirus E1B proteins  Autophagy  Apoptosis
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