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王斌,白伟,郭甲瑞.臭氧氧化预处理减轻大鼠脊髓缺血再灌注损伤[J].脊柱外科杂志,2019,17(1):56-59.
臭氧氧化预处理减轻大鼠脊髓缺血再灌注损伤     点此下载全文 (Fulltext)
王斌  白伟  郭甲瑞
焦作市第二人民医院骨科, 焦作 454001
基金项目:
DOI:10.3969/j.issn.1672-2957.2019.01.012
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摘要:
      目的 探讨臭氧氧化预处理对大鼠脊髓缺血再灌注损伤的作用及可能机制。方法 将36只健康成年雄性SD大鼠随机分为假手术组、模型组和臭氧预处理组,每组12只。模型组和臭氧预处理组采用夹闭右肾动脉上腹主动脉的方法构建大鼠脊髓缺血再灌注损伤模型,臭氧预处理大鼠于建模前7 d开始以1 mg(/kg·d)腹腔注射臭氧(50 mg/L)-氧气混合气体至建模。分别于恢复灌流后6、12、24和48 h时,对各组大鼠后肢神经运动功能进行评分。末次评分后取脊髓标本,行HE染色观察组织病理学变化,行2,3,5-氯化三苯基四氮唑(TTC)染色检测缺血面积,用试剂盒检测丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平。结果 与假手术组相比,模型组和臭氧预处理组大鼠恢复灌流后6、12、24、48 h神经运动功能评分均降低,差异均有统计学意义(P<0.05);与模型组相比,臭氧预处理组大鼠恢复灌流后6、12、24、48 h神经运动功能评分升高,差异有统计学意义(P<0.05)。与假手术组相比,模型组和臭氧预处理组大鼠脊髓组织缺血面积增大,差异均有统计学意义(P<0.05);与模型组相比,臭氧预处理组大鼠脊髓缺血面积减小,差异有统计学意义(P<0.05)。与假手术组相比,模型组和臭氧预处理组大鼠脊髓组织中MDA水平升高,而SOD和GSH-Px水平降低,差异均有统计学意义(P<0.05);与模型组相比,臭氧预处理组大鼠脊髓组织中MDA水平降低,而SOD和GSH-Px水平升高,差异均有统计学意义(P<0.05)。结论 臭氧预处理可有效减轻大鼠脊髓缺血再灌注损伤,其机制可能与抑制脊髓组织氧化应激反应有关。
关键词:脊髓  再灌注损伤  基因表达  臭氧  氧化性应激  大鼠
Ozone oxidative preconditioning reducing spinal cord ischemia-reperfusion injury in rats    Fulltext
WANG Bin  BAI Wei  GUO Jia-rui
Department of Orthopaedics, Second People's Hospital of Jiaozuo, Jiaozuo 454001, Henan, China
Fund Project:
Abstract:
      Objective To investigate the effect of ozone oxidative preconditioning on spinal cord ischemia-reperfusion injury in rats and its mechanisms. Methods Thirty-six healthy adult male SD rats were randomly divided into sham operation group, model group and ozone preconditioning group, with 12 rats in each group. Rat spinal cord ischemia-reperfusion injury model was constructed in the model group and ozone preconditioning group, and the rats in the ozone preconditioning group were intraperitoneally injected with 1 mg(/kg·d) ozone-oxygen mixed gas (ozone 50 mg/L) from 7 d before modeling. At 6, 12, 24 and 48 h after reperfusion, the neurological function of hind limbs in each group was scored, respectively. After the last scoring for neurological function, the histopathological changes of the spinal cord were observed through HE staining in each group, and the ischemic area was measured through 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected using available kit. Results Compared with the sham operation group, the neurological function scores at 6, 12, 24, 48 h in the model and ozone preconditioning groups decreased with statistically significant differences (P<0.05); compared with the model group, the neurological function scores at 6, 12, 24, 48 h in the ozone preconditioning group increased with statistically significant differences (P<0.05). The ischemic areas of spinal cord in the model and ozone preconditioning groups were higher than the sham operation group with statistically significant differences (P<0.05), and the ischemic area in the ozone preconditioning group was lower than the model group with statistically significant difference(P<0.05). Compared with the sham operation group, the levels of MDA in the spinal cord tissues in the model and ozone preconditioning groups increased, while the levels of SOD and GSH-Px decreased, with statistically significant differences(P<0.05); compared with the model group, the level of MDA in the spinal cord tissue decreased in the ozone preconditioning, while the levels of SOD and GSH-Px increased, with statistically significant differences (P<0.05). Conclusion Ozone preconditioning could effectively reduce the spinal cord ischemia-reperfusion injury in rats. The mechanism might be related to inhibition of oxidative stress in spinal cord tissue.
Keywords:Spinal cord  Reperfusion injury  Gene expression  Ozone  Oxidative stress  Rats
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