| 贺云飞,王爽,马俊,余将明,席炎海,叶晓健.纳米纱/多孔纳米纤维支架的生物相容性[J].脊柱外科杂志,2019,17(3):198-202,206. |
纳米纱/多孔纳米纤维支架的生物相容性  点此下载全文 (Fulltext) |
| 贺云飞1 2△ 王爽1△ 马俊1 余将明1 席炎海1 叶晓健1* |
1. 海军军医大学附属长征医院骨科, 上海 200003;
2. 解放军联勤保障部队940医院骨科, 兰州 730070 |
| 基金项目：国家自然科学基金面上项目（81472071；81772445） |
| DOI：10.3969/j.issn.1672-2957.2019.03.010 |
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| 摘要： |
| 目的 通过体内外实验评价纳米纱/多孔纳米纤维材料的生物相容性，为构建仿生组织工程纤维环及进一步开展体内实验奠定基础。方法 通过静电纺丝技术制备取向纳米纤维支架（AFS）、取向纳米纱支架（AYS）、三维多孔纳米纤维支架（3DPS）。将大鼠骨髓间充质干细胞（BMSC）接种于3种支架，静态培养，观察细胞在支架上黏附、生长和增殖情况，评价支架的体外生物相容性。将3种支架植入大鼠皮下，在不同时间点取材，通过HE染色、异物巨细胞的形态数目及炎性因子基因表达量评价支架的体内生物相容性。结果 体外实验表明BMSC在3种支架上均能黏附、增殖，生长良好。体内实验表明大鼠皮下植入3种支架后早期（1~2周）均有轻度炎性反应，4周后炎性细胞浸润减少，异物巨细胞数量逐渐减少。炎性因子基因表达水平与炎性细胞浸润程度相符。结论 AYS和3DPS具有良好的生物相容性，可用于进一步体内实验研究。 |
| 关键词：椎间盘 生物相容性材料 纳米医学 |
| Biocompatibility of nano-yarn/porous nano-fibers scaffolds Fulltext |
| HE Yun-fei1 2△ WANG Shuang1△ MA Jun1 YU Jiang-ming1 XI Yan-hai1 YE Xiao-jian1* |
1. Department of Orthopaedics, Changzheng Hospital, Navy Medical University, Shanghai 200003, China;
2. Department of Orthopaedics, No. 940 Hospital of PLA Joint Logistic Support Force, Lanzhou 730070, Gansu, China |
| Fund Project: |
| Abstract: |
| Objective To evaluate the biocompatibility of nano-yarn/porous nano-fibrous materials in vitro and in vivo,so as to lay the foundation for the fabrication of bionic anulus fibrosus and in vivo experiments in future. Methods Aligned nanofibrous scaffold(AFS),aligned nanoyarn scaffold(AYS) and three-dimensional porous nanofibrous scaffold(3DPS) were prepared by electrospinning technology. Bone marrow mesenchymal stem cells(BMSC) were seeded into the 3 scaffolds. After static culture,the adhesion,growth and proliferation of BMSCs in scaffold materials were observed,and the biocompatibility of the scaffolds in vitro was evaluated. The 3 scaffolds were implanted subcutaneously into rats and samples were taken at different time points. The biocompatibility of the 3 scaffolds in vivo was evaluated by HE staining,observing and counting foreign body giant cells and inflammatory cytokines gene expression. Results In vitro experiments showed that BMSCs can adhere,proliferate and grow well in the 3 scaffolds. In vivo experiments showed that the 3 scaffolds had mild inflammatory reaction at early stage(1-2 weeks). After 4 weeks,the infiltration of inflammatory cells decreased and the number of foreign giant cells gradually decreased. The expression level of inflammatory cytokines was consistent with inflammatory cell infiltration. Conclusion The AYS and 3DPS have good biocompatibility,and can be used for further study. |
| Keywords:Intervertebral disc Biocompatible materials Nanomedicine |
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