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冉仁国,张岱阳,李贺伟.Ⅰ型骨质疏松症患者长链非编码RNA WT1-AS与骨密度关系[J].脊柱外科杂志,2024,22(3):158-163.
Ⅰ型骨质疏松症患者长链非编码RNA WT1-AS与骨密度关系     点此下载全文 (Fulltext)
冉仁国1  张岱阳1*  李贺伟2
1. 恩施土家族苗族自治州中心医院脊柱外科, 恩施 445000;
2. 华中科技大学同济医学院附属梨园医院骨科, 武汉 430077
基金项目:武汉中青年医学骨干人才培养工程(2019年)
DOI:10.3969/j.issn.1672-2957.2024.03.003
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摘要:
      目的 探讨Ⅰ型骨质疏松症(OP)患者血清长链非编码RNA(lncRNA)WT1-AS水平与骨密度(BMD)及骨代谢指标的相关性。 方法 纳入2018年9月—2020年9月恩施土家族苗族自治州中心医院收治的女性Ⅰ型OP患者108例(OP组),同期绝经后骨量减少者114例(骨量减少组)以及同期年龄相符的绝经后非OP女性120例(对照组)。采用实时荧光定量聚合酶链式反应(qRT-PCR)测定研究对象血清lncRNA WT1-AS水平,采用酶联免疫法测定骨代谢指标,包括血清骨钙素(OCN)、β-胶原降解产物(β-CTX)和Ⅰ型原胶原N端前肽(PINP)。采用Pearson法分析OP患者血清lncRNA WT1-AS与BMD及骨代谢指标相关性,采用logistic回归法分析影响OP发生的因素;采用受试者工作特征(ROC)曲线分析血清lncRNA WT1-AS对OP的诊断价值。沉默大鼠前成骨细胞的lncRNA WT1-AS表达,观察lncRNA WT1-AS对前成骨细胞分化和矿化的作用。 结果 与对照组和骨量减少组相比,OP组OCN水平降低,β-CTX、PINP水平和lncRNA WT1-AS相对表达量升高,差异均有统计学意义(P<0.05)。OP患者血清lncRNA WT1-AS水平与BMD(L1~4和股骨颈)、OCN水平呈负相关(r=-0.542、-0.557、-0.608),与β-CTX、PINP水平呈正相关(r=0.576、0.595)。血清lncRNA WT1-AS水平是影响OP发生的危险因素。血清lncRNA WT1-AS水平诊断OP的ROC曲线下面积(AUC)为0.796,特异度为92.11%,灵敏度为62.96%。沉默大鼠前成骨细胞的lncRNA WT1-AS表达,可促进矿化结节生成。 结论 Ⅰ型OP患者血清lncRNA WT1-AS异常高表达与BMD、骨代谢有关,且影响Ⅰ型OP的发生,对OP具有一定的诊断价值。
关键词:骨质疏松  RNA,长链非编码  WT1蛋白质
Relationship between long noncoding RNA WT1-AS and bone mineral density in patients with typeⅠosteoporosis    Fulltext
Ran Renguo1  Zhang Daiyang1*  Li Hewei2
1. Department of Spinal Surgery, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, Hubei, China;
2. Department of Orthopaedics, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, Hubei, China
Fund Project:
Abstract:
      Objective To investigate the correlation of serum long noncoding RNA(lncRNA) WT1-AS level with bone mineral density(BMD) and bone metabolism indexes in patients with typeⅠosteoporosis(OP).Methods From September 2018 to September 2020,108 female patients with typeⅠOP(OP group),114 women with postmenopausal osteopenia(osteopenia group),and 120 postmenopausal women without OP(control group) treated in Central Hospital of Enshi Tujia and Miao Autonomous Prefecture were admitted. Real time fluorescence quantitative polymerase chain reaction(qRT-PCR) was used to measure the serum lncRNA WT1-AS levels,and enzyme-linked immunosorbent assay was used to measure bone metabolism indicators,including serum osteocalcin(OCN),β-collagen degradation product(β-CTX),and typeⅠprocollagen N-terminal propeptide(PINP). Pearson's method was used to analyze the correlation between serum lncRNA WT1-AS and BMD in OP patients,and logistic regression was used to analyze the factors affecting the occurrence of OP. The diagnostic value of serum lncRNA WT1-AS for OP was analyzed using receiver operating characteristic(ROC) curve. The expression of lncRNA WT1-AS in rat osteoblasts was silenced,and the effect of lncRNA WT1-AS on the differentiation and mineralization of osteoblasts was observed. Results Compared with the control group and osteopenia groups,OP group had lower OCN level,and higher serum β-CTX,PINP levels and relative expression of lncRNA WT1-AS,and the differences were statistically significant(P<0.05). The serum lncRNA WT1-AS level in OP patients was negatively correlated with BMD(L1-4 and femoral neck) and OCN(r=-0.542,-0.557,-0.608),and positively correlated with β-CTX and PINP levels(r=0.576,0.595). The serum lncRNA WT1-AS level was the risk factor affecting the occurrence of OP. The area under the ROC curve(AUC) of serum lncRNA WT1-AS level for diagnosing OP was 0.796,with the specificity of 92.11% and sensitivity of 62.96%. The silencing of lncRNA WT1-AS expression in rat osteoblasts promoted the formation of mineralized nodules. Conclusion The abnormally high expression of serum lncRNA WT1-AS in typeⅠOP patients is related to BMD and bone metabolism,and affects the occurrence of typeⅠOP,which has a certain diagnostic value for OP.
Keywords:Osteoporosis  RNA,long noncoding  WT1 proteins
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